International Academic Journal of Innovative Research

  • ISSN 2454-390X

Utilization of the Acetone solution of α-Pinene and pine needles of Pinus sylvestris (L) for the quality improvement of cocoons and silk fibres spinned by fifth instar larvae of silkworm, Bombyx mori (L) (Race : PM x CSR2)

Vitthalrao B. Khyade, Vishakha S. Chape and Priyanka B. Ghadge

Abstract: Insect juvenoid activity of Terpenes and terpene contents of Pine needles of Pinus sylvestris (L) are well established facts. Topical application of ten microliters of one milligram per liter strength acetone solution of α-Pinene and Pine needles to the individual fifth instar larvae of multivoltine cross breed race of silkworm, Bombyx mori ( L ) ( PM x CSR2) at 48, 54, 60 and 66 hours after the fourth moult was found variously reflected into the prolongation of larval duration (17.011– 45.394 % for α-Pinene treated groups and 19.078 – 48.684 % for Pine needle treated groups); improvement in the tissue somatic index (TSI) of silk glands (3.054 – 3.344 for α-Pinene treated groups and 3.071 – 3.094 % for Pine needle treated groups); cocoon shell ratio (2.791 – 3.179 for α-Pinene treated groups and 2.694 – 3.631 for Pine needle treated groups) and denier scale of silk filament (0.333 – 2.359 for α-Pinene treated group and 0.466 – 2.405 for Pine needle treated group). Acetone solution of α-Pinene and acetone solution of pine needles, thus chiefly lengthening the larval duration (age) in silkworm, Bombyx mori (L). The lengthened larval life is sufficient to prove the juvenoid activity of acetone solution of Pine Needles. Treated group of larvae may be utilizing the lengthened life span for the fortification of cocoon shell through the silk filament. The synergistic activity of limonene and Pine needles in the present attempt is hypothesized to be due to changes in the membrane fluidity, interference with membrane bound signaling proteins and cell cycle arrest. Efficient utilization of α-Pinene and pine needles, through acetone solvent for topical application to the fifth instared larvae of silkworm, Bombyx mori (L) may open a new innovative biotechnological avenue in the sericulture industries

Keywords: Limonene; Pine needles; TSI ; Shell Ratio; Denier Scale of Silk Filament

Page: 1-19

DOI: 10.9756/IAJIR/V6I1/1910001

Volume 6, Issue 1, 2019